Karyotype analysis of Alocasia cucullata (Lour.) Schott using fluorochrome banding and fluorescence in situ hybridization with rDNA probes
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منابع مشابه
Karyotype analysis and visualization of 45S rRNA genes using fluorescence in situ hybridization in aroids (Araceae)
Karyotype analysis and FISH mapping using 45S rDNA sequences on 6 economically important plant species Anthuriumandraeanum Linden ex André, 1877, Monsteradeliciosa Liebmann, 1849, Philodendronscandens Koch & Sello, 1853, Spathiphyllumwallisii Regel, 1877, Syngoniumauritum (Linnaeus, 1759) Schott, 1829 and Zantedeschiaelliottiana (Knight, 1890) Engler, 1915 within the monocotyledonous family Ara...
متن کاملMulticolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii.
The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the most extensively used methods in molecular cytogenetics producing chromosome landmarks, fluorescen...
متن کاملThe chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding.
In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x = 18), Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus x Poncirus hybrids (2n = 2x = 18). Counterstaining with the fluorochromes chromomycin A3 and DAPI uniquely identi...
متن کاملComparative Study of Fluorescence in situ Hybridization Analysis and Karyotype Analysis in Spontaneous Abortion Etiology Diagnosis
Objective To compare the roles of fluorescence in situ hybridization (FISH) and karyotype analysis on chorionic villus in spontaneous abortion. Methods A total of 201 cases were included in this study and were randomly divided into 2 groups by the same gestational age. The villi from 100 cases (group A) were cultured and used for karyotype analysis, while the uncultured villi from 101 women (gr...
متن کاملKaryotype refinement by multicolor fluorescence in situ hybridization analysis in 18 patients with acute lymphoblastic leukemia.
Eighteen patients with acute lymphoblastic leukemia (ALL) were investigated using spectral karyotyping (SKY), and multicolor fluorescence in situ hybridization (FISH) banding analyses. SKY analysis confirmed previous results in patients with G-banded diploid karyotype. Nevertheless in three out of five patients with two or more chromosomal abnormalities, SKY and multicolor banding methods refin...
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ژورنال
عنوان ژورنال: Caryologia
سال: 2016
ISSN: 0008-7114,2165-5391
DOI: 10.1080/00087114.2016.1155922